Structure of the oxygen adduct intermediate in the bacterial luciferase reaction: C nuclear magnetic resonance determination.
نویسندگان
چکیده
By using FMN enriched in (13)C (90%) at position C-4a, we have conclusively shown that the reaction of molecular oxygen with bacterial luciferase-bound FMNH(2) forms an adduct at the 4a position. Consistent with this are (13)C NMR studies of FMN and other flavin compounds which show that this carbon should be unusually reactive in the reduced 1,5-dihydroflavins with respect to electrophilic attacks.
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 75 12 شماره
صفحات -
تاریخ انتشار 1978